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SUMMARY:Establishment of robust salivary gland-specific expression in tran
 sgenic anopheline mosquito - Shigeto Yoshida (Kanazawa University School o
 f Pharmacy\, Japan)
DTSTART;VALUE=DATE-TIME:20151120T153000Z
DTEND;VALUE=DATE-TIME:20151120T163000Z
UID:https://new.talks.ox.ac.uk/talks/id/54bcbb85-cc75-4be5-8426-8c4c0d3bec
 c6/
DESCRIPTION:Recent advances in the genetically engineering of Anopheline m
 osquitoes have raised hopes for their use as new strategies for malaria co
 ntrol and provided a powerful tool to investigate parasite-mosquito intera
 ctions. The interruption of the parasite cycle in the mosquito using trans
 genic strategies can occur at the midgut level\, interfering with ookinete
  invasion or oocyst differentiation\, and at the salivary gland level\, bl
 ocking invasion of gland cells or the transmission of infective sporozoite
 s from the salivary glands. So far\, only the carboxypeptidase and vitello
 genine promoters have been used for driving transgenes in mosquitoes for t
 he gut- and fat body- specific expression\, respectively. Therefore\, rese
 arch on transmission blockade using transgenic mosquitoes has been limited
  to the midgut stage of the parasites.\n\nIn the seminar\, Shigeto Yoshida
  will present the development of transgenic Anopheles stephensi mosquitoes
  that specifically express Discosoma sp. red fluorescent protein (DsRed) i
 n their salivary glands. Using advanced 3D confocal microscopy\, robust ex
 pression of DsRed in the distal-lateral lobes of the salivary glands is ob
 served\, where the sporozoites invade preferentially\, driven by a newly i
 solated salivary gland-specific promoter in a living mosquito as well as i
 n dissected salivary glands. Furthermore\, by using GFP-expressing sporozo
 ites\, Shigeto Yoshida will also show the parasites passing through the sa
 livary glands from the outer surface. These results open up the possibilit
 y of elucidating the process and molecules involved in the salivary glands
 -parasites interactions\, and may lead to the development of transmission-
 blocking strategies by using genetically modified mosquitoes that express 
 anti-parasitic genes in a salivary gland-specific manner.\n\nSpeakers:\nSh
 igeto Yoshida (Kanazawa University School of Pharmacy\, Japan)
LOCATION:Basement Seminar Room\, ORCRB\, Headington\, OX3 7DQ
TZID:Europe/London
URL:https://new.talks.ox.ac.uk/talks/id/54bcbb85-cc75-4be5-8426-8c4c0d3bec
 c6/
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DESCRIPTION:Talk:Establishment of robust salivary gland-specific expressio
 n in transgenic anopheline mosquito - Shigeto Yoshida (Kanazawa University
  School of Pharmacy\, Japan)
TRIGGER:-PT1H
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