BEGIN:VCALENDAR VERSION:2.0 PRODID:talks.ox.ac.uk BEGIN:VEVENT SUMMARY:"Licensed to kill: Post-translational activation of an antibacteri al effector protein" - Prof. Christopher Hayes (MCDB faculty at UC Santa B arbara) DTSTART;VALUE=DATE-TIME:20230901T100000 DTEND;VALUE=DATE-TIME:20230901T110000 UID:https://new.talks.ox.ac.uk/talks/id/eaf0ad62-3d50-424d-97d6-884b5ddcfd 11/ DESCRIPTION:\nSpeakers:\nProf. Christopher Hayes (MCDB faculty at UC Santa Barbara) LOCATION:Dorothy Crowfoot Hodgkin Building (Phase 2 Seminar Room 20-138)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/eaf0ad62-3d50-424d-97d6-884b5ddcfd 11/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:"Licensed to kill: Post-translational activation of an an tibacterial effector protein" - Prof. Christopher Hayes (MCDB faculty at U C Santa Barbara) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:"Licensed to kill: Post-translational activation of an antibacteri al effector protein" - Prof. Christopher Hayes (MCDB faculty at UC Santa B arbara) DTSTART;VALUE=DATE-TIME:20230901T100000 DTEND;VALUE=DATE-TIME:20230901T110000 UID:https://new.talks.ox.ac.uk/talks/id/eaf0ad62-3d50-424d-97d6-884b5ddcfd 11/ DESCRIPTION:\nSpeakers:\nProf. Christopher Hayes (MCDB faculty at UC Santa Barbara) LOCATION:Dorothy Crowfoot Hodgkin Building (Phase 2 Seminar Room 20-138)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/eaf0ad62-3d50-424d-97d6-884b5ddcfd 11/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:"Licensed to kill: Post-translational activation of an an tibacterial effector protein" - Prof. Christopher Hayes (MCDB faculty at U C Santa Barbara) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:The coevolution of host selectivity in the microbiome - Connor Sha rp (University of Oxford) DTSTART;VALUE=DATE-TIME:20190617T110000 DTEND;VALUE=DATE-TIME:20190617T120000 UID:https://new.talks.ox.ac.uk/talks/id/cbe42161-5c08-44bf-9c92-3bc981e60d e1/ DESCRIPTION:\nSpeakers:\nConnor Sharp (University of Oxford) LOCATION:Dorothy Crowfoot Hodgkin Building (Main Seminar Room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/cbe42161-5c08-44bf-9c92-3bc981e60d e1/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:The coevolution of host selectivity in the microbiome - C onnor Sharp (University of Oxford) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:The coevolution of host selectivity in the microbiome - Connor Sha rp (University of Oxford) DTSTART;VALUE=DATE-TIME:20190617T110000 DTEND;VALUE=DATE-TIME:20190617T120000 UID:https://new.talks.ox.ac.uk/talks/id/cbe42161-5c08-44bf-9c92-3bc981e60d e1/ DESCRIPTION:\nSpeakers:\nConnor Sharp (University of Oxford) LOCATION:Dorothy Crowfoot Hodgkin Building (Main Seminar Room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/cbe42161-5c08-44bf-9c92-3bc981e60d e1/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:The coevolution of host selectivity in the microbiome - C onnor Sharp (University of Oxford) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:‘The Type VII secretion system in the human pathogen Staphylococ cus aureus’ - Professor Tracy Palmer\, FRS (Professor of Microbiology\, Institute for Cell and Molecular Biosciences\, Newcastle University) DTSTART;VALUE=DATE-TIME:20181122T160000Z DTEND;VALUE=DATE-TIME:20181122T170000Z UID:https://new.talks.ox.ac.uk/talks/id/dde37fc9-1515-4f62-8717-ae0c84062b 42/ DESCRIPTION:\nSpeakers:\nProfessor Tracy Palmer\, FRS (Professor of Microb iology\, Institute for Cell and Molecular Biosciences\, Newcastle Universi ty) LOCATION:Dorothy Crowfoot Hodgkin Building (Main Seminar Room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/dde37fc9-1515-4f62-8717-ae0c84062b 42/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:‘The Type VII secretion system in the human pathogen St aphylococcus aureus’ - Professor Tracy Palmer\, FRS (Professor of Microb iology\, Institute for Cell and Molecular Biosciences\, Newcastle Universi ty) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:Structure and Assembly of an antibacterial speargun: the Type VI s ecretion system - Dr. Eric Cascales (Laboratoire d'Ingenierie des Systemes Macromoleculaires\, IBSM\, CNRS\, France) DTSTART;VALUE=DATE-TIME:20171117T160000Z DTEND;VALUE=DATE-TIME:20171117T170000Z UID:https://new.talks.ox.ac.uk/talks/id/38370caa-430a-4f51-a20e-902d61152e 5a/ DESCRIPTION:\nSpeakers:\nDr. Eric Cascales (Laboratoire d'Ingenierie des S ystemes Macromoleculaires\, IBSM\, CNRS\, France) LOCATION:Dorothy Crowfoot Hodgkin Building (Main Seminar Room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/38370caa-430a-4f51-a20e-902d61152e 5a/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:Structure and Assembly of an antibacterial speargun: the Type VI secretion system - Dr. Eric Cascales (Laboratoire d'Ingenierie des Systemes Macromoleculaires\, IBSM\, CNRS\, France) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:Early developmental program shapes colony morphology in bacteria - Gideon Mamou (Hebrew University of Jerusalem\, Israel) DTSTART;VALUE=DATE-TIME:20170120T120000Z DTEND;VALUE=DATE-TIME:20170120T130000Z UID:https://new.talks.ox.ac.uk/talks/id/e4f18b9b-daa8-4c1d-baff-63e0580780 6b/ DESCRIPTION:\nSpeakers:\nGideon Mamou (Hebrew University of Jerusalem\, Is rael) LOCATION:Dorothy Crowfoot Hodgkin Building (Main seminar room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/e4f18b9b-daa8-4c1d-baff-63e0580780 6b/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:Early developmental program shapes colony morphology in b acteria - Gideon Mamou (Hebrew University of Jerusalem\, Israel) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:MSB seminar - Professor Colin Kleanthous (Department of Biochemist ry\, University of Oxford)\, Axel Nystrom DTSTART;VALUE=DATE-TIME:20170109T110000Z DTEND;VALUE=DATE-TIME:20170109T120000Z UID:https://new.talks.ox.ac.uk/talks/id/1eff60e6-17af-4c5a-9c7c-0df1f38759 8d/ DESCRIPTION:\nSpeakers:\nProfessor Colin Kleanthous (Department of Biochem istry\, University of Oxford)\, Axel Nystrom LOCATION:Dorothy Crowfoot Hodgkin Building (Main Seminar Room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/1eff60e6-17af-4c5a-9c7c-0df1f38759 8d/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:MSB seminar - Professor Colin Kleanthous (Department of B iochemistry\, University of Oxford)\, Axel Nystrom TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:Protein antibiotic import in bacteria - Professor Colin Kleanthous (Department of Biochemistry\, University of Oxford) DTSTART;VALUE=DATE-TIME:20170424T130000 DTEND;VALUE=DATE-TIME:20170424T140000 UID:https://new.talks.ox.ac.uk/talks/id/22bf6511-044f-4fcc-8c9c-9b535f2f90 4c/ DESCRIPTION:\nSpeakers:\nProfessor Colin Kleanthous (Department of Biochem istry\, University of Oxford) LOCATION:Dorothy Crowfoot Hodgkin Building (Main seminar room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/22bf6511-044f-4fcc-8c9c-9b535f2f90 4c/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:Protein antibiotic import in bacteria - Professor Colin K leanthous (Department of Biochemistry\, University of Oxford) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:"Intrinsic disorder controls the function of p53 and other cancer- associated IDPs" - Gary Daughdrill (University of South Florida) DTSTART;VALUE=DATE-TIME:20160707T130000 DTEND;VALUE=DATE-TIME:20160707T140000 UID:https://new.talks.ox.ac.uk/talks/id/4dcb6cbe-d876-4da5-aa4f-038b4f2484 65/ DESCRIPTION:p53 is a tumor suppressor and cell cycle regulator that is act ivated by protein-protein interactions and posttranslational modifications (PTMs). Deletion or mutation of p53 can dramatically increase susceptibil ity to cancer. p53 is also an intrinsically disordered protein (IDP). IDPs are highly dynamic\, do not form stable tertiary structures\, and contain variable amounts of transient secondary structure. IDP domains are hotspo ts for PTMs and they frequently mediate protein-protein interactions throu gh coupled folding and binding. IDP domains that interact with other prote ins can contain defined levels of transient secondary structure that resem ble their complex-bound structure11. These levels of residual structure ca n modulate binding affinities with other proteins by tuning the change in conformational entropy that occurs during the coupled folding and binding reaction. We recently showed that levels of residual helicity in the disor dered p53 transcriptional activation domain (p53TAD) controlled the bindin g affinity to the E3 ubiquitin ligase Mdm2\, both in vitro and inside livi ng cells. The levels of residual helicity in free p53TAD were controlled b y conserved prolines flanking the Mdm2 binding site. Mutating these prolin es to alanine resulted in higher p53TAD helicity and stronger Mdm2 binding . This stronger Mdm2 binding inhibits phosphorylation of p53 and leads dir ectly to more rapid degradation of p53 following DNA damage. Lower levels of p53 reduce target gene expression and prevent cell cycle arrest. Our re sults indicate that precise levels of intrinsic disorder and residual heli city are necessary for regulating the p53-signaling network and changing t he levels of disorder will modify the effects of phosphorylation and other PTMs. We are currently investigating whether changing the levels of disor der for other cancer associated IDPs like the myeoblastosis transcription factor family member\, cMyb\, and the mixed lineage leukemia protein\, MLL \, control their function. \nSpeakers:\nGary Daughdrill (University of Sou th Florida) LOCATION:Dorothy Crowfoot Hodgkin Building (Biochemistry Seminar Room)\, o ff South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/4dcb6cbe-d876-4da5-aa4f-038b4f2484 65/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:"Intrinsic disorder controls the function of p53 and othe r cancer-associated IDPs" - Gary Daughdrill (University of South Florida) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:"Intrinsic disorder controls the function of p53 and other cancer- associated IDPs" - Gary Daughdrill (University of South Florida) DTSTART;VALUE=DATE-TIME:20160707T130000 DTEND;VALUE=DATE-TIME:20160707T140000 UID:https://new.talks.ox.ac.uk/talks/id/4dcb6cbe-d876-4da5-aa4f-038b4f2484 65/ DESCRIPTION:p53 is a tumor suppressor and cell cycle regulator that is act ivated by protein-protein interactions and posttranslational modifications (PTMs). Deletion or mutation of p53 can dramatically increase susceptibil ity to cancer. p53 is also an intrinsically disordered protein (IDP). IDPs are highly dynamic\, do not form stable tertiary structures\, and contain variable amounts of transient secondary structure. IDP domains are hotspo ts for PTMs and they frequently mediate protein-protein interactions throu gh coupled folding and binding. IDP domains that interact with other prote ins can contain defined levels of transient secondary structure that resem ble their complex-bound structure11. These levels of residual structure ca n modulate binding affinities with other proteins by tuning the change in conformational entropy that occurs during the coupled folding and binding reaction. We recently showed that levels of residual helicity in the disor dered p53 transcriptional activation domain (p53TAD) controlled the bindin g affinity to the E3 ubiquitin ligase Mdm2\, both in vitro and inside livi ng cells. The levels of residual helicity in free p53TAD were controlled b y conserved prolines flanking the Mdm2 binding site. Mutating these prolin es to alanine resulted in higher p53TAD helicity and stronger Mdm2 binding . This stronger Mdm2 binding inhibits phosphorylation of p53 and leads dir ectly to more rapid degradation of p53 following DNA damage. Lower levels of p53 reduce target gene expression and prevent cell cycle arrest. Our re sults indicate that precise levels of intrinsic disorder and residual heli city are necessary for regulating the p53-signaling network and changing t he levels of disorder will modify the effects of phosphorylation and other PTMs. We are currently investigating whether changing the levels of disor der for other cancer associated IDPs like the myeoblastosis transcription factor family member\, cMyb\, and the mixed lineage leukemia protein\, MLL \, control their function. \nSpeakers:\nGary Daughdrill (University of Sou th Florida) LOCATION:Dorothy Crowfoot Hodgkin Building (Biochemistry Seminar Room)\, o ff South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/4dcb6cbe-d876-4da5-aa4f-038b4f2484 65/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:"Intrinsic disorder controls the function of p53 and othe r cancer-associated IDPs" - Gary Daughdrill (University of South Florida) TRIGGER:-PT1H END:VALARM END:VEVENT BEGIN:VEVENT SUMMARY:O-GlcNAc signalling in development and disease - Professor Daan va n Aalten\, FRSE (Wellcome Trust Senior Fellow\, MRC Protein Phosphorylatio n Unit and Division of Molecular Microbiology\, University of Dundee) DTSTART;VALUE=DATE-TIME:20160425T130000 DTEND;VALUE=DATE-TIME:20160425T140000 UID:https://new.talks.ox.ac.uk/talks/id/1113e29f-0bee-4473-a226-1364c675e8 44/ DESCRIPTION:\nSpeakers:\nProfessor Daan van Aalten\, FRSE (Wellcome Trust Senior Fellow\, MRC Protein Phosphorylation Unit and Division of Molecular Microbiology\, University of Dundee) LOCATION:Dorothy Crowfoot Hodgkin Building (Main Meeting Room)\, off South Parks Road OX1 3QU TZID:Europe/London URL:https://new.talks.ox.ac.uk/talks/id/1113e29f-0bee-4473-a226-1364c675e8 44/ BEGIN:VALARM ACTION:display DESCRIPTION:Talk:O-GlcNAc signalling in development and disease - Professo r Daan van Aalten\, FRSE (Wellcome Trust Senior Fellow\, MRC Protein Phosp horylation Unit and Division of Molecular Microbiology\, University of Dun dee) TRIGGER:-PT1H END:VALARM END:VEVENT END:VCALENDAR