Full-length single-cell RNA-sequencing: From short to long-reads

This is a hybrid event - with the speaker attending in-person and viewable on Teams.

Single-cell RNA-sequencing (scRNA-seq) methods have revolutionized the way scientists study the cell transcriptome. In this field, full-length scRNA-seq protocols stand as the gold- standard for sensitivity. After testing >400 reaction conditions, we developed FLASH-seq, the fastest and most sensitive scRNA-seq protocol currently protocol available. In our most recent work, we push the boundaries of this protocol to study and quantify isoforms, leveraging the recent advances in long-read sequencing technologies. The result is FLASH-seq-ONT, a method that combines the high-sensitivity of plate-based methods with molecule counting and true isoform characterization on the Oxford Nanopore Technologies sequencing platform.