Full-length single-cell RNA-sequencing: From short to long-reads
This is a hybrid event - with the speaker attending in-person and viewable on Teams.
Single-cell RNA-sequencing (scRNA-seq) methods have revolutionized the way scientists study the cell transcriptome. In this field, full-length scRNA-seq protocols stand as the gold- standard for sensitivity. After testing >400 reaction conditions, we developed FLASH-seq, the fastest and most sensitive scRNA-seq protocol currently protocol available. In our most recent work, we push the boundaries of this protocol to study and quantify isoforms, leveraging the recent advances in long-read sequencing technologies. The result is FLASH-seq-ONT, a method that combines the high-sensitivity of plate-based methods with molecule counting and true isoform characterization on the Oxford Nanopore Technologies sequencing platform.
Date: 22 January 2024, 12:00 (Monday, 2nd week, Hilary 2024)
Venue: MRC Weatherall Institute of Molecular Medicine, Headington OX3 9DS
Venue Details: Seminar Room
Speaker: Vincent Hahaut (Institute of Molecular and Clinical Ophthalmology Basel, Switzerland)
Organising department: MRC Weatherall Institute of Molecular Medicine
Organiser: Yasmine Saito (Weatherall Institute, University of Oxford)
Host: Maria Greco (MRC Weatherall Institute of Molecular Medicine)
Booking required?: Recommended
Booking email: seminar.admin@imm.ox.ac.uk
Audience: Members of the University only
Editor: Yasmine Saito