From real-time to pseudotime: Tracking B cell fate decisions across time and space

Special visiting speaker seminar

Our lab is interested in how and where antibody responses are made, particularly in the context of vaccination and autoimmunity. We track B cell responses in real-time by intravital two-photon microscopy to visualise cell fates and single cell RNA sequencing to determine the molecules that drives these cell fate decisions. Here I will share some of our data tracking the fates of T follicular helper cells and memory B cells and their reactivation to generate antibody-secreting plasma cells in the secondary antibody response.
Tri graduated from medical school at the University of Sydney and trained as a clinical immunologist and immunopathologist at the Royal Prince Alfred Hospital, Sydney. He did his PhD with Prof. Tony Basten and Dr Robert Brink at the Centenary Institute where he developed the SWHEL model for tracking in vivo B cell responses. Tri undertook post-doctoral studies at UCSF with Dr Jason Cyster where he learnt intravital two-photon microscopy from Dr Taka Okada and Dr Andrew Bullen. He has since returned to the Garvan Institute where he uses two-photon microscopy and single cell sequencing to track B cell fates in the setting of vaccinations and autoimmunity in both mouse models and the patients he sees in the clinic.